Carbonic Anhydrase III Human Recombinant, His Tag ( CAIII Human, His )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

Supplied in 10mM Tris-HCl (pH 8), 250mM NaCl, 0.5mM DTT, 1.5mM Cysteine, and 50% Glycerol.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Carbonic anhydrase III Human Recombinant produced in E.Coli, and having a molecular mass of 33.9 kDa. CAIII is expressed with an amino-terminal hexahistidine tag.The CA-III is purified by proprietary chromatographic techniques.

Protein Background

Carbonic anhydrase (carbonate dehydratase) is a family of metalloenzymes (enzymes that contain one or more metal atoms as a functional component of the enzyme) that catalyze the rapid (and reversible) conversion of carbon dioxide to bicarbonate and proton

Expression host

Escherichia Coli.

Synonyms

Car3, CAIII, Carbonic anhydrase 3, EC 4.2.1.1, Carbonic anhydrase III, Carbonate dehydratase III, CA-III.

Reagent Appearance

Sterile Filtered blue solution.

Stability

CAIII although stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Antibody Properties

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Cytotoxic and staining antibody. For staining, use 10ul/1,000,000 cells. Titer for cytotoxicity should be determined by the investigator.

Immunological function

This antibody is only available conjugated to Biotin and FITC.