Cyclophilin-D Human Recombinant ( Cyclophilin D Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

Cyclophilin-D 0.2um filtered solution containing PBS pH7.4, 1mM DTT and 10% glycerol.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Cyclophilin-D Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 178 amino acids and having a molecular mass of 18.9kDa.The Cyclophilin-D is purified by proprietary chromatographic techniques.

Protein Background

Cyclophilin-D is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and speeds up the protein folding. Cyclophilin-D possess PPIase activity an

Expression host

Escherichia Coli.

Synonyms

Peptidylprolyl isomerase D, PPID, CYPD, CYP-40, 40 kDa peptidyl-prolyl cis-trans isomerase, PPIase, Rotamase, Cyclophilin-40, CYP40, Cyclophilin-related protein, MGC33096, EC 5.2.1.8.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS.

Biological Activity

Specific activity is > 250 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH8.0 using chymotrypsin.

Antibody Properties

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By intracellular staining of Dengue-infected cells, 1:1000 dilution.

Application And Suggested Dilutions

Minimal cross-reactivity with other Dengue subtypes.