Epiregulin Human Recombinant ( EREG Human )

Data sheet

Formulation

Epiregulin was lyophilized from 0.5mg/ml solution ciontaing 20mM PBS buffer pH-7.4 containing 130mM sodium chloride.

Solubility

It is recommended to reconstitute the lyophilized Epiregulin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Epiregulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6 kDa. Epiregulin is purified by proprietary chromatographic techniques.

Protein Background

Epiregulin is part of the EGF family. Epiregulin functions as a ligand of EGFR, as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors.Epiregulin is expressed mostly in the placenta and peripheral b

Expression host

Escherichia Coli.

Synonyms

EREG, Epiregulin, ER.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Epiregulin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Epiregulin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended

Amino acid sequence

MVAQVSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL.

Biological Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is 2.0 ng/ml, corresponding to a specific activity of > 500,000 units/mg.