Protein Kinase A holoenzyme type II alpha Recombinant ( PKACa2-RIIa2 )

Catalog # PT_41769

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$201.00
DescriptionInactive holoenzyme consisting of one dimeric regulatory subunit type II alpha and two monomeric catalytic subunits (cAMP-free). Protein Kinase A is purified by proprietary chromatographic techniques.SourceEscherichia Coli.Physical
Product Size: 1ug
Quantity

Data sheet

Formulation
PKA holoenzyme type-II alpha is supplied in 50% glycerol.
Purity
Greater than 95% as determined by SDS-PAGE.
Applications
The product is suitable for analysing PKA type II agonists (cAMP analogs) or antagonists.
Description
Inactive holoenzyme consisting of one dimeric regulatory subunit type II alpha and two monomeric catalytic subunits (cAMP-free). Protein Kinase A is purified by proprietary chromatographic techniques.
Protein Background
The protein kinase A holoenzyme is a heterotetramer composed of two types of subunits: Catalytic and Regulatory. The Catalyticsubunit contains the enzyme's active site. It also contains a domain that binds ATP and a domain that binds the regulatory subuni
Expression host
Escherichia Coli.
Synonyms
Protein Kinase A holoenzyme type II alpha, PKACa2-RIIa2.
Reagent Appearance
Sterile Filtered clear solution.
Stability
PKA should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C. Avoid multiple freeze-thaw cycles.
Biological Activity
Holoenzyme can be activated by adding the second messenger cAMP (Activation constant about 100nM) releasing two monomeric catalytic subunits.
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