Trefoil Factor-3 Human Recombinant, His Tag ( TFF3 Human, His )

Data sheet

Formulation

The TFF3 protein was lyophilized from 0.4m filtered solution at a concentration of 0.5mg/mL containing 20mM Tris pH-7.5 and 150mM NaCl.

Solubility

Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.

Purity

Greater than 95.0% as by SDS-PAGE.

Description

TFF3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids (22-80 a.a.) which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 7.82 kDa. TFF3 Human Recombin

Protein Background

Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which prote

Expression host

Escherichia Coli.

Synonyms

TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Reagent Appearance

White lyophilized (freeze-dried) powder.

Stability

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca

Amino acid sequence

MKHHHHHHAS EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF.