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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| topoisomerase(DNA) II alpha 170kDa | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha(1466-1480aa RKPSTSDDSDSNFEK), different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TOP2A |
Protein | DNA topoisomerase 2-alpha |
Uniprot ID | P11388 |
Function | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the type II topoisomerase family. |
Aliases | alpha isozyme antibody|ATP hydrolyzing DNA topoisomerase II alfa antibody|DNA gyrase antibody|DNA topoisomerase 2 alpha antibody|DNA topoisomerase 2-alpha antibody|DNA topoisomerase II 170 kD antibody|DNA topoisomerase II alpha isozyme antibody|DNA topoisomerase II antibody|DNA Topoisomerase2 antibody|TOP 2A antibody|TOP2 antibody|TOP2A antibody|TOP2A_HUMAN antibody|TopII alpha antibody|Topo II alpha antibody|Topoisomerase DNA II alpha 170kDa antibody|Topoisomerase II alpha 170 kDa antibody|TP2A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Topoisomerase II alpha antibody, ASA-B1896, Western blotting
All lanes: Anti Topoisomerase II alpha (ASA-B1896) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 174KD
Observed bind size: 174KD
All lanes: Anti Topoisomerase II alpha (ASA-B1896) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 174KD
Observed bind size: 174KD
