More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
tumor necrosis factor receptor superfamily, member 1B | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNF Receptor II(38-55aa YECQISQEYYDRKAQMCC), different from the related rat sequence by three amino acids. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFRSF1B |
Protein | Tumor necrosis factor receptor superfamily member 1B |
Uniprot ID | P25119 |
Function | Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | |
Aliases | CD 120b antibody|CD120b antibody|CD120b antigen antibody|Etanercept antibody|p75 antibody|p75TNFR antibody|p80 TNF alpha receptor antibody|p80 TNF-alpha receptor antibody|TBP-2 antibody|TBP2 antibody|TBPII antibody|TNF R II antibody|TNF R2 antibody|TNF R75 antibody|TNF-R2 antibody|TNF-RII antibody|TNFBR antibody|TNFR 2 antibody|TNFR-II antibody|TNFR2 antibody|TNFR75 antibody|TNFR80 antibody|TNFRII antibody|TNFRSF 1B antibody|TNFRSF1B antibody|TNR1B antibody|TNR1B_HUMAN antibody|Tumor necrosis factor beta receptor antibody|Tumor necrosis factor binding protein 2 antibody|Tumor necrosis factor receptor 2 antibody|Tumor necrosis factor receptor superfamily member 1B precursor antibody|Tumor necrosis factor receptor type II antibody|Tumor necrosis factor-binding protein 2 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TNF Receptor II antibody, ASA-B1888, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: HEPA Cell Lysate