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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor receptor superfamily, member 1B | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TNF Receptor II (288-318aa KKKPLCLQREAKVPHLPADKARGTQGPEQQH), different from the related mouse sequence by eleven amino acids, and from the related rat sequence by ten amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNFRSF1B |
Protein | Tumor necrosis factor receptor superfamily member 1B |
Uniprot ID | P20333 |
Function | Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
Tissue Specificity | |
Sub-cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities | Contains 4 TNFR-Cys repeats. |
Aliases | CD 120b antibody|CD120b antibody|CD120b antigen antibody|Etanercept antibody|p75 antibody| p75TNFR antibody|p80 TNF alpha receptor antibody|p80 TNF-alpha receptor antibody|TBP-2 antibody|TBP2 antibody|TBPII antibody|TNF R II antibody|TNF R2 antibody|TNF R75 antibody| TNF-R2 antibody|TNF-RII antibody| TNFBR antibody|TNFR 2 antibody|TNFR-II antibody|TNFR2 antibody| TNFR75 antibody|TNFR80 antibody|TNFRII antibody|TNFRSF 1B antibody| TNFRSF1B antibody|TNR1B antibody|TNR1B_HUMAN antibody|Tumor necrosis factor beta receptor antibody| Tumor necrosis factor binding protein 2 antibody| Tumor necrosis factor receptor 2 antibody| Tumor necrosis factor receptor superfamily member 1B precursor antibody|Tumor necrosis factor receptor type II antibody| Tumor necrosis factor-binding protein 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TNF Receptor II antibody, ASA-B1889, Western blotting
All lanes: Anti TNF Receptor II (ASA-B1889) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
All lanes: Anti TNF Receptor II (ASA-B1889) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
