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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lymphotoxin alpha(TNF superfamily, member 1) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TNF beta(45-59aa AQTARQHPKMHLAHS). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LTA |
Protein | Lymphotoxin-alpha(LT-alpha) |
Uniprot ID | P01374 |
Function | Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. |
Tissue Specificity | |
Sub-cellular localization | Secreted. Membrane. Note: The homotrimer is secreted. The heterotrimer is membrane-associated. |
Sequence Similarities | Belongs to the tumor necrosis factor family. |
Aliases | LTalpha antibody|DIF antibody|LT alpha antibody|LT antibody|LT-alpha antibody|LTA antibody|LTalpha antibody|Lymphotoxin alpha(TNF superfamily, member 1) antibody|Lymphotoxin alpha antibody|Lymphotoxin-alpha antibody|TNF B antibody|TNF superfamily member 1 antibody|TNF-beta antibody|TNFB antibody|TNFB_HUMAN antibody|TNFbeta antibody|TNFSF1 antibody|Tumor necrosis factor beta antibody|Tumor necrosis factor ligand superfamily member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TNF beta antibody, ASA-B1884, All Western blotting
All lanes: Anti-TNF beta(ASA-B1884) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti-TNF beta(ASA-B1884) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
