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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| tumor necrosis factor | Polyclonal | IgG | Rabbit | Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived rat TNF alpha recombinant protein (Position: D89-L235). Rat TNF alpha shares 95% amino acid (aa) sequence identity with mouse TNF alpha. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TNF |
Protein | Tumor necrosis factor |
Uniprot ID | P16599 |
Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Single-pass type II membrane protein . |
Sequence Similarities | |
Aliases | APC1 antibody|APC1 protein antibody|Cachectin antibody|DIF antibody|Differentiation inducing factor antibody|Macrophage cytotoxic factor antibody|MCF antibody|Necrosin antibody|TNF a antibody|TNF alpha antibody|TNF antibody|TNF Macrophage Derived antibody|TNF Monocyte Derived antibody|TNF Superfamily Member 2 antibody|TNF superfamily, member 2 antibody|TNF, macrophage derived antibody|TNF, monocyte derived antibody|TNF-a antibody|TNF-alpha antibody|TNFA antibody|TNFA_HUMAN antibody|TNFSF2 antibody|Tumor necrosis factor alpha antibody|Tumor necrosis factor antibody|Tumor necrosis factor ligand superfamily member 2 antibody|Tumor Necrosis Factor Precursor antibody|Tumor Necrosis Factor, Membrane Form antibody|Tumor necrosis factor, soluble form antibody|Tumour Necrosis Factor Alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TNF alpha antibody, ASA-B1882--1.jpg
All lanes: Anti-TNF alpha(ASA-B1882) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Kidney Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
All lanes: Anti-TNF alpha(ASA-B1882) at 0.5ug/ml
Lane 1: PC-12 Whole Cell Lysate at 40ug
Lane 2: Rat Spleen Tissue Lysate at 40ug
Lane 3: Rat Brain Tissue Lysate at 40ug
Lane 4: Rat Kidney Tissue Lysate at 40ug
Lane 5: Rat Liver Tissue Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
