More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| anoctamin 1, calcium activated chloride channel | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A(927-943aa KVLMVELFMREEQDKQQ), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANO1 |
Protein | Anoctamin-1 |
Uniprot ID | Q5XXA6 |
Function | Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. |
Tissue Specificity | Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cytoplasm. Note: Cytoplasmic localization seen in neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors. |
Sequence Similarities | |
Aliases | ANO 1 antibody|ANO1 antibody|ANO1_HUMAN antibody|Anoctamin 1 antibody|Anoctamin 1 calcium activated chloride channel antibody|Anoctamin-1 antibody|Anoctamin1 antibody|Discovered on gastrointestinal stromal tumors protein 1 antibody|DOG 1 antibody|FLJ10261 antibody|Membrane protein antibody|Oral cancer overexpressed 2 antibody|Oral cancer overexpressed protein 2 antibody|ORAOV 2 antibody|ORAOV2 antibody|TAOS 2 antibody|TAOS2 antibody|TMEM 16A antibody|TMEM16A antibody|Transmembrane protein 16A(eight membrane spanning domains) antibody|Transmembrane protein 16A antibody|Tumor amplified and overexpressed sequence 2 antibody|Tumor-amplified and overexpressed sequence 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TMEM16A antibody, ASA-B1880, All Western blotting
All lanes: Anti-ANO1(ASA-B1880) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Predicted bind size: 114KD
Observed bind size: 114KD
All lanes: Anti-ANO1(ASA-B1880) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Predicted bind size: 114KD
Observed bind size: 114KD
