More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| toll-like receptor 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human TLR2(364-379aa YLDLSENLMVEEYLKN), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TLR2 |
Protein | Toll-like receptor 2 |
Uniprot ID | O60603 |
Function | Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. |
Tissue Specificity | Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. |
Sub-cellular localization | Membrane ; Single-pass type I membrane protein . |
Sequence Similarities | Belongs to the Toll-like receptor family. |
Aliases | CD282 antibody|CD282 antigen antibody|TIL 4 antibody|TIL4 antibody|TLR 2 antibody|TLR2 antibody|TLR2_HUMAN antibody|Toll like receptor 2 antibody|Toll like receptor 2 precursor antibody|Toll-like receptor 2 antibody|Toll/interleukin 1 receptor like 4 antibody|Toll/interleukin 1 receptor like protein 4 antibody|Toll/interleukin receptor like protein 4 antibody|Toll/interleukin-1 receptor-like protein 4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TLR2 antibody, ASA-B1872, Western blotting
Lane 1: U937 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 1: U937 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
