More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| toll-like receptor 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TLR1(771-786aa NLRAAINIKLTEQAKK). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TLR1 |
Protein | Toll-like receptor 1 |
Uniprot ID | Q15399 |
Function | Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). |
Tissue Specificity | Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. |
Sub-cellular localization | Cell membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, phagosome membrane ; Single-pass type I membrane protein . |
Sequence Similarities | Belongs to the Toll-like receptor family. |
Aliases | CD281 antibody|CD281 antigen antibody|KIAA0012 antibody|LPRS5 antibody|rsc786 antibody|Similar to Drosophila Toll protein antibody|TIL antibody|TIL. LPRS5 antibody|Tlr1 antibody|TLR1_HUMAN antibody|Toll(Drosophila) homolog antibody|Toll like receptor 1 antibody|Toll like receptor 1 Precursor antibody|Toll-like receptor 1 antibody|Toll/Interleukin 1 receptor like antibody|Toll/Interleukin 1 receptor-like antibody|Toll/interleukin-1 receptor-like protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TLR1 antibody, ASA-B1870, Western blotting
All lanes: Anti TLR1 (ASA-B1870) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
All lanes: Anti TLR1 (ASA-B1870) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Lane 6: PANC Whole Cell Lysate at 40ug
Predicted bind size: 90KD
Observed bind size: 90KD
