More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| TIMP metallopeptidase inhibitor 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP3(198-211aa WAPPDKSIINATDP), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TIMP3 |
Protein | Metalloproteinase inhibitor 3 |
Uniprot ID | P35625 |
Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix. |
Sequence Similarities | Belongs to the protease inhibitor I35 (TIMP) family. |
Aliases | HSMRK222 antibody|K222 antibody|K222TA2 antibody|Metalloproteinase inhibitor 3 antibody|MIG 5 protein antibody|MIG5 protein antibody|Protein MIG 5 antibody|Protein MIG-5 antibody|SFD antibody|Sorsby fundus dystrophy pseudoinflammatory antibody|TIMP 3 antibody|TIMP metallopeptidase inhibitor 3 antibody|TIMP-3 antibody|TIMP3 antibody|TIMP3_HUMAN antibody|Tissue Inhibitor of Metalloproteinase 3 antibody|Tissue inhibitor of metalloproteinases 3 antibody|Tissue inhibitor of metalloproteinases3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human Mouse, Rat | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TIMP3 antibody, ASA-B1864, Western blotting
Lane 1: Recombinant Human TIMP3 Protein 10ng
Lane 2: Recombinant Human TIMP3 Protein 5ng
Lane 3: Recombinant Human TIMP3 Protein 2.5ng
Lane 1: Recombinant Human TIMP3 Protein 10ng
Lane 2: Recombinant Human TIMP3 Protein 5ng
Lane 3: Recombinant Human TIMP3 Protein 2.5ng
