More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| DNA nucleotidylexotransferase | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DNTT |
Protein | DNA nucleotidylexotransferase |
Uniprot ID | P04053 |
Function | Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the DNA polymerase type-X family. |
Aliases | Deoxynucleotidyltransferase terminal antibody|DNA nucleotidylexotransferase antibody|DNTT antibody|Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody|TDT antibody|TDT_HUMAN antibody|Terminal addition enzyme antibody|Terminal deoxynucleotidyltransferase antibody|Terminal deoxyribonucleotidyltransferase antibody|Terminal transferase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TdT antibody, ASA-B1841--1.jpg
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: Recombinant Human TDT Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: Recombinant Human TDT Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-TdT antibody, ASA-B1841--2.jpg
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
All lanes: Anti TDT (ASA-B1841) at 0.5ug/ml
WB: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
