More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| TATA box binding protein | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetiA synthetic peptide corresponding to a sequence in the middle region of human TBP(99-127aa AAAVQQSTSQQATQGTSGQAPQLFHSQTL), different from the related mouse sequence by four amino acids.c peptide corresponding to a sequence at the N-terminus of h | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TBP |
Protein | TATA-box-binding protein |
Uniprot ID | P20226 |
Function | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. |
Tissue Specificity | Widely expressed, with levels highest in the testis and ovary. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | GTF2D antibody|GTF2D1 antibody|HDL4 antibody|MGC117320 antibody| MGC126054 antibody| MGC126055 antibody|SCA17 antibody|TATA binding factor antibody|TATA box factor antibody| TATA sequence binding protein antibody|TATA sequence-binding protein antibody|TATA-binding factor antibody|TATA-box binding protein N-terminal domain antibody|TATA-box factor antibody|TATA-box-binding protein antibody|TBP antibody|TBP_HUMAN antibody|TFIID antibody| Transcription initiation factor TFIID TBP subunit antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TBP antibody, ASA-B1838, Western blotting
All lanes: Anti TBP (ASA-B1838) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Lane 4: Mouse Ovary Tissue Lysate at 50ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti TBP (ASA-B1838) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Lane 4: Mouse Ovary Tissue Lysate at 50ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
