More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| T-box 21 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet(522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TBX21 |
Protein | T-box transcription factor TBX21 |
Uniprot ID | Q9UL17 |
Function | Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. |
Tissue Specificity | T-cell specific. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | |
Aliases | T bet antibody|T box 21 antibody|T box expressed in T cells antibody|T box protein 21 antibody|T box transcription factor TBX21 antibody|T cell specific T box transcription factor antibody|T cell specific T box transcription factor T bet antibody|T PET antibody|T-box protein 21 antibody|T-box transcription factor TBX21 antibody|T-cell-specific T-box transcription factor T-bet antibody|TBET antibody|TBLYM antibody|TBX 21 antibody|TBX21 antibody|TBX21_HUMAN antibody|TPET antibody|Transcription factor TBLYM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-T-bet/Tbx21 antibody, ASA-B1826, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TBX21,34.7KD(162aa tag+ V381-N535)
Lane 1: Recombinant Human TBX21 Protein 5ng
Lane 2: Recombinant Human TBX21 Protein 2.5ng
Lane 3: Recombinant Human TBX21 Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human TBX21,34.7KD(162aa tag+ V381-N535)
Lane 1: Recombinant Human TBX21 Protein 5ng
Lane 2: Recombinant Human TBX21 Protein 2.5ng
Lane 3: Recombinant Human TBX21 Protein 1.25ng
