More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| talin 1; talin 2 | Monoclonal | IgG | Mouse | Bovine, Human | WB | Ascites |
Immunogen |
| Purified chicken gizzard talin. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | TLN1; TLN2 |
Protein | Talin-1; Talin-2 |
Uniprot ID | Human(Q9Y490), Mouse(P26039), Rat(Q498D4); Human(Q9Y4G6), Mouse(Q71LX4), Rat(D3ZA84) |
Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell surface . Cell junction, focal adhesion . Note: Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity). |
Sequence Similarities | Contains 1 FERM domain. |
Aliases | TLN|KIAA1027|ILWEQ|TALIN |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 2-4μg/ml | Human, mouse, rat, chicken | AssaySolutio's ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
