More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cell adhesion molecule 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SynCAM recombinant protein (Position: Q45-P340). Human SynCAM shares 99% amino acid (aa) sequence identity with mouse SynCAM. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CADM1 |
Protein | Cell adhesion molecule 1 |
Uniprot ID | Q9BY67 |
Function | Mediates homophilic cell-cell adhesion in a Ca(2+)- independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the nectin family. |
Aliases | BL2 antibody|Cadm1 antibody|CADM1_HUMAN antibody|Cell adhesion molecule 1 antibody|DKFZp686F1789 antibody|IgSF4 antibody|IGSF4A antibody|Immunoglobulin superfamily member 4 antibody|MGC149785 antibody|MGC51880 antibody|NECL-2 antibody|NECL2 antibody|Nectin like 2 antibody|Nectin-like protein 2 antibody|RA175 antibody|SgIGSF antibody|Spermatogenic immunoglobulin superfamily antibody|ST17 antibody|sTSLC 1 antibody|Synaptic cell adhesion molecule antibody|SynCAM antibody|SynCAM1 antibody|TSLC-1 antibody|TSLC1 antibody|Tumor suppressor in lung cancer 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SynCAM antibody, ASA-B1821--1.jpg
All lanes: Anti SYNCAM (ASA-B1821) at 0.5ug/ml
WB: Human Recombinant SYNCAM Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti SYNCAM (ASA-B1821) at 0.5ug/ml
WB: Human Recombinant SYNCAM Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-SynCAM antibody, ASA-B1821--2.jpg
All lanes: Anti SYNCAM (ASA-B1821) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti SYNCAM (ASA-B1821) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
