More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| synaptopodin | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptopodin (629-658aa EKPKVTPNPDLLDLVQTADEKRRQRDQGEV), different from the related mouse sequence by one amino acid, and identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SYNPO |
Protein | Synaptopodin |
Uniprot ID | Q8N3V7 |
Function | Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity (By similarity). |
Tissue Specificity | Expressed in cerebral cortex. |
Sub-cellular localization | Cytoplasm, cytoskeleton . Cell junction, tight junction . Perikaryon . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell junction, synapse . Note: Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Associated with dendritic spines of a subset of synapses (By similarity). |
Sequence Similarities | Belongs to the synaptopodin family. |
Aliases | KIAA1029 antibody|Synaptopodin antibody|SYNPO antibody|SYNPO_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Synaptopodin antibody, ASA-B1819, Western blotting
All lanes: Anti Synaptopodin (ASA-B1819) at 0.5ug/ml
Lane 1: Mosue Brain Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
All lanes: Anti Synaptopodin (ASA-B1819) at 0.5ug/ml
Lane 1: Mosue Brain Tissue Lysate at 50ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
