More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| baculoviral IAP repeat containing 5 | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | BIRC5 |
Protein | Baculoviral IAP repeat-containing protein 5 |
Uniprot ID | O70201 |
Function | Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7 (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm . Nucleus . Chromosome. Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Midbody . Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (By similarity). |
Sequence Similarities | Belongs to the IAP family. |
Aliases | API4 antibody|Apoptosis Inhibitor 4 antibody|Apoptosis inhibitor survivin antibody|Apoptosis inhibitor4 antibody|Baculoviral IAP repeat containing 5 antibody|Baculoviral IAP repeat containing 5 antibody|Baculoviral IAP repeat containing protein 5 antibody|Baculoviral IAP repeat-containing protein 5 antibody|BIRC 5 antibody|BIRC5 antibody|BIRC5_HUMAN antibody|EPR 1 antibody|IAP4 antibody|Survivin variant 3 alpha antibody|SVV antibody|TIAP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Survivin antibody, ASA-B1816, Western blotting
All lanes: Anti Survivin (ASA-B1816) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Predicted bind size: 16KD
Observed bind size: 16KD
All lanes: Anti Survivin (ASA-B1816) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Predicted bind size: 16KD
Observed bind size: 16KD
