More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystatin B(stefin B) | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse Stefin B(62-77aa DKCVHLRVFQPLPHEN), different from the related rat sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CSTB |
Protein | Cystatin-B |
Uniprot ID | Q62426 |
Function | This is an intracellular thiol proteinase inhibitor. |
Tissue Specificity | Widely expressed. Highest expression in heart, liver and kidney. Lower levels in brain, lung and skeletal muscle. Lowest levels in spleen and testis. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | CHROW21 antibody|CPI B antibody|CPI-B antibody|CST6 antibody|CSTB antibody|Cystatin B antibody|Cystatin-B antibody|CYTB antibody|CYTB_HUMAN antibody|EPM1 antibody|EPM1A antibody|Liver thiol proteinase inhibitor antibody|PME antibody|Stefin-B antibody|STFB antibody|ULD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- StefinB antibody, ASA-B1804, Western blotting
All lanes: Anti StefinB (ASA-B1804) at 0.5ug/ml
WB: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti StefinB (ASA-B1804) at 0.5ug/ml
WB: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 11KD
Observed bind size: 11KD
