More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| signal transducer and activator of transcription 5A | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a(735-751aa YNMYPQNPDHVLDQDGE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | STAT5A |
Protein | Signal transducer and activator of transcription 5A |
Uniprot ID | P42229 |
Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the transcription factor STAT family. |
Aliases | Mammary gland factor antibody|MGF antibody|Signal transducer and activator of transcription 5A antibody|STA5A_HUMAN antibody|STAT 5 antibody|STAT 5A antibody|STAT5 antibody|STAT5A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-STAT5a antibody, ASA-B1797, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: CEM Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: CEM Cell Lysate
