More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| stanniocalcin 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Stanniocalcin 1(150-167aa QLPNHFSNRYYNRLVRSL), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | STC1 |
Protein | Stanniocalcin-1 |
Uniprot ID | P52823 |
Function | Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. |
Tissue Specificity | Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | Stanniocalcin antibody|Stanniocalcin-1 antibody|STC antibody|STC-1 antibody|Stc1 antibody|STC1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Stanniocalcin 1 antibody, ASA-B1787, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: Rat Stomach Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: SW620 Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Lane 3: Rat Stomach Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5: SW620 Cell Lysate
