More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| suppression of tumorigenicity 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human ST5(571-586aa KRHSHDDMLLLAQLSL), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ST5 |
Protein | Suppression of tumorigenicity 5 protein |
Uniprot ID | P78524 |
Function | Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP- bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth. |
Tissue Specificity | Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level). |
Sub-cellular localization | |
Sequence Similarities | Contains 1 dDENN domain. |
Aliases | DENN domain containing protein 2B antibody|DENN domain-containing protein 2B antibody|DENN/MADD domain containing 2B antibody|DENND2B antibody|HeLa tumor suppression 1 antibody|HTS1 antibody|p126 antibody|ST 5 antibody|ST5 antibody|Suppression of tumorigenicity 5 antibody|Suppression of tumorigenicity 5 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ST5 antibody, ASA-B1786, All Western blotting
All lanes: Anti-ST5(ASA-B1786) at0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: A431 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: NIH Whole Cell Lysate at 40ug
Predicted bind size: 126KD
Observed bind size: 126KD
All lanes: Anti-ST5(ASA-B1786) at0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 40ug
Lane 2: A431 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: NIH Whole Cell Lysate at 40ug
Predicted bind size: 126KD
Observed bind size: 126KD
