More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| nuclear receptor coactivator 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human SRC3(1043-1061aa NLEGQSDERALLDQLHTLL), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | NCOA3 |
Protein | Nuclear receptor coactivator 3 |
Uniprot ID | Q9Y6Q9 |
Function | Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. |
Tissue Specificity | Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney. |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus. |
Sequence Similarities | Belongs to the SRC/p160 nuclear receptor coactivator family. |
Aliases | ACTR antibody|AIB 1 antibody|AIB-1 antibody|AIB1 antibody|Amplified in breast cancer 1 antibody||Amplified in breast cancer 1 protein antibody|bHLHe42 antibody|CAGH 16 antibody|CAGH16 antibody|CBP interacting protein antibody|CBP-interacting protein antibody|Class E basic helix-loop-helix protein 42 antibody|CTG 26 antibody|CTG26 antibody|KAT13B / AIB1 antibody|MGC141848 antibody|NCOA 3 antibody|NCoA-3 antibody|NCOA3 antibody|Nuclear receptor coactivator 3 antibody|pCIP antibody|RAC 3 antibody|RAC-3 antibody|RAC3 antibody|Receptor associated coactivator 3 antibody|Receptor-associated coactivator 3 antibody|SRC 3 antibody|SRC-3 antibody|SRC3 antibody|Steroid receptor coactivator protein 3 antibody|Thyroid hormone receptor activator molecule 1 antibody|TNRC14 antibody|TNRC16 antibody|TRAM 1 antibody|TRAM-1 antibody|TRAM1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SRC3 antibody, ASA-B1781, All Western blotting
All lanes: Anti-NCOA3(ASA-B1781) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 155KD
Observed bind size: 155KD
All lanes: Anti-NCOA3(ASA-B1781) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 155KD
Observed bind size: 155KD
