More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SRY (sex determining region Y)-box 4 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SOX4 (1-31aa MVQQTNNAENTEALLAGESSDSGAGLELGIA), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOX4 |
Protein | Transcription factor SOX-4 |
Uniprot ID | Q06945 |
Function | Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif. |
Tissue Specificity | Testis, brain, and heart. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 HMG box DNA-binding domain. |
Aliases | Ecotropic viral integration site 16 antibody|EVI 16 antibody|EVI16 antibody|Sox4 antibody|SOX4_HUMAN antibody|SRY (sex determining region Y) box 4 antibody|SRY box containing gene 4 antibody|SRY related HMG box gene 4 antibody|Transcription factor SOX-4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SOX4 antibody, ASA-B1760, Western blotting
All lanes: Anti SOX4 (ASA-B1760) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti SOX4 (ASA-B1760) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 47KD
