More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SRY (sex determining region Y)-box 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of Human SOX1(215-248aa QHPGAGGAHPHAHPAHPHPHHPHAHPHNPQPMHR), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOX1 |
Protein | Transcription factor SOX-1 |
Uniprot ID | O00570 |
Function | Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). |
Tissue Specificity | Mainly expressed in the developing central nervous system. |
Sub-cellular localization | Nucleus . |
Sequence Similarities | |
Aliases | Sex determining region Y box 1 antibody|sox1 antibody|SOX1_HUMAN antibody| SRY antibody|SRY box containing gene 1 antibody|SRY related HMG box gene 1 antibody|Transcription factor Sox-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SOX1 antibody, ASA-B1758, Western blotting
All lanes: Anti SOX1 (ASA-B1758) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti SOX1 (ASA-B1758) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
