More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| superoxide dismutase 1, soluble | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1 (116-146aa RTLVVHEKADDLGKGGNEESTKTGNAGSRLA), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOD1 |
Protein | Superoxide dismutase [Cu-Zn] |
Uniprot ID | P00441 |
Function | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Nucleus. Note: Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. |
Sequence Similarities | Belongs to the Cu-Zn superoxide dismutase family. |
Aliases | ALS 1 antibody|ALS antibody|ALS1 antibody|Amyotrophic lateral sclerosis 1 adult antibody|Amyotrophic lateral sclerosis 1 antibody|Cu/Zn SOD antibody|Cu/Zn superoxide dismutase antibody|Homodimer antibody|hSod1 antibody| Indophenoloxidase A antibody|IPOA antibody|Mn superoxide dismutase antibody| SOD 1 antibody|SOD antibody|SOD soluble antibody|SOD1 antibody|SODC antibody|SODC_HUMAN antibody|Superoxide dismutase [Cu Zn] antibody| Superoxide dismutase [Cu-Zn] antibody|Superoxide dismutase 1 antibody| Superoxide dismutase 1 soluble antibody|Superoxide dismutase antibody| Superoxide dismutase Cu Zn antibody|Superoxide dismutase cystolic antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SOD1 antibody, ASA-B1750, Western blotting
All lanes: Anti SOD1 (ASA-B1750) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 16KD
All lanes: Anti SOD1 (ASA-B1750) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 16KD
