More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| suppressor of cytokine signaling 4 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SOCS4 recombinant protein (Position: M291-C440). Human SOCS4 shares 98% amino acid (aa) sequence identity with mouse SOCS4. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOCS4 |
Protein | Suppressor of cytokine signaling 4 |
Uniprot ID | Q8WXH5 |
Function | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. Substrate-recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits EGF signaling by mediating the degradation of the Tyr-phosphorylated EGF receptor/EGFR. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Contains 1 SH2 domain. |
Aliases | CIS4 antibody|Cish4 antibody|Cytokine inducible SH2 protein 4 antibody| DKFZp686J1568 antibody|HSPC060 antibody|SH2 domain containing SOCS box protein antibody|SOCS-4 antibody|SOCS-7 antibody|SOCS4 antibody|SOCS4 _ HUMAN antibody|SOCS6 antibody|SOCS7 antibody|SSI4 antibody|STAI4 antibody| STAT induced STAT inhibitor 4 antibody|STAT4 antibody|STATI4 antibody| Suppressor of cytokine signaling 4 antibody|Suppressor of cytokine signaling 7 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SOCS4 antibody, ASA-B1749, Western blotting
All lanes: Anti SOCS4 (ASA-B1749) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
All lanes: Anti SOCS4 (ASA-B1749) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 51KD
Observed bind size: 51KD
