More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| suppressor of cytokine signaling 3 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SOCS3 recombinant protein (Position: V2-L225). Human SOCS3 shares 96.9% and 95.5% amino acid (aa) sequence identity with mouse and rat SOCS3, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SOCS3 |
Protein | Suppressor of cytokine signaling 3 |
Uniprot ID | O14543 |
Function | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity). |
Tissue Specificity | Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. |
Sub-cellular localization | |
Sequence Similarities | Contains 1 SH2 domain. |
Aliases | ATOD4 antibody|CIS 3 antibody|CIS-3 antibody|CIS3 antibody|Cish3 antibody| Cytokine induced SH2 protein 3 antibody|Cytokine-inducible SH2 protein 3 antibody|E2a Pbx1 target gene in fibroblasts 10 antibody|EF 10 antibody| MGC71791 antibody|SOCS 3 antibody|SOCS-3 antibody|Socs3 antibody| SOCS3_HUMAN antibody|SSI 3 antibody|SSI-3 antibody|SSI3 antibody|STAT induced STAT inhibitor 3 antibody|STAT-induced STAT inhibitor 3 antibody| Suppressor of cytokine signaling 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SOCS3 antibody, ASA-B1748, Western blotting
All lanes: Anti SOCS3 (ASA-B1748) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Mouse Thymus Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti SOCS3 (ASA-B1748) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Mouse Thymus Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 25KD
Observed bind size: 25KD
