More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
snail family zinc finger 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence in the middle region of human SNAI3 (155-178aa FHCHKPYHTLAGLARHRQLHCHLQ), different from the related mouse sequence by two amino acids. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SNAI3 |
Protein | Zinc finger protein SNAI3 |
Uniprot ID | Q3KNW1 |
Function | Seems to inhibit myoblast differentiation. Transcriptional repressor of E-box-dependent transactivation of downstream myogenic bHLHs genes. Binds preferentially to the canonical E-box sequences 5'-CAGGTG-3' and 5'-CACCTG-3' (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Nucleus . |
Sequence Similarities | Belongs to the snail C2H2-type zinc-finger protein family. |
Aliases | MGC129606 antibody|Protein snail homolog 3 antibody|SMUC antibody|SNAI3 antibody|SNAI3_ HUMAN antibody|Snail homolog 3 (Drosophila) antibody|SNAIL3 antibody|Zfp293 antibody|Zinc finger protein 293 antibody|Zinc finger protein SNAI3 antibody|ZNF293 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SNAI3 antibody, ASA-B1737, Western blotting
All lanes: Anti SNAI3 (ASA-B1737) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 32KD