More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| SMAD family member 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SMAD6 recombinant protein (Position: E131-D456). Human SMAD6 shares 95.3% amino acid (aa) sequence identity with mouse SMAD6. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SMAD6 |
Protein | Mothers against decapentaplegic homolog 6 |
Uniprot ID | O43541 |
Function | Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions. |
Tissue Specificity | Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the dwarfin/SMAD family. |
Aliases | hSMAD6 antibody|HsT17432 antibody|MAD (mothers against decapentaplegic Drosophila) homolog 6 antibody|MAD homolog 6 antibody|MAD mothers against decapentaplegic homolog 6 (Drosophila) antibody|MADH6 antibody|MADH7 antibody|Mothers against decapentaplegic drosophila homolog of 6 antibody| Mothers against decapentaplegic homolog 6 antibody|Mothers against DPP homolog 6 antibody|SMAD 6 antibody|SMAD family member 6 antibody|SMAD mothers against DPP homolog 6 antibody|Smad6 antibody|SMAD6_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SMAD6 antibody, ASA-B1735, Western blotting
All lanes: Anti SMAD6 (ASA-B1735) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
All lanes: Anti SMAD6 (ASA-B1735) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Mouse Lung Tissue Lysate at 50ug
Lane 3: Human Placenta Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
