SLC12A6 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
solute carrier family 12(potassium/chloride transporters), member 6	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SLC12A6(1060-1080aa QKAKSMEGFQDLLNMRPDQSN), different from the related mouse and rat sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SLC12A6
Protein	Solute carrier family 12 member 6
Uniprot ID	Q9UHW9
Function	Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
Tissue Specificity	Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
Sub-cellular localization	Basolateral cell membrane ; Multi-pass membrane protein .
Sequence Similarities
Aliases	ACCPN antibody|Furosemide sensitive KCl cotransporter 3 antibody|Gaxp antibody|KCC 3 antibody|KCC 3A antibody|KCC 3B antibody|KCC3 A antibody|KCC3 antibody|KCC3 B antibody|KCC3A antibody|KCC3B antibody|Potassium chloride cotransporter 3 antibody|Potassium chloride cotransporter KCC3a S3 antibody|SLC12 A6 antibody|SLC12A 6 antibody|Solute carrier family 12(potassium/chloride transporters), member 6 antibody|Solute carrier family 12, member 6 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human Mouse, Rat	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information