More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sirtuin 3 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SIRT3 |
Protein | NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
Uniprot ID | Q9NTG7 |
Function | NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Mitochondrion matrix. |
Sequence Similarities | Belongs to the sirtuin family. Class I subfamily. |
Aliases | hSIRT 3 antibody|hSIRT3 antibody|Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody|NAD dependent deacetylase sirtuin 3 mitochondrial antibody|NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody|Regulatory protein SIR2 homolog 3 antibody|Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody|Sir 2 like 3 antibody|SIR 2 like protein 3 antibody|SIR 3 antibody|SIR2 L3 antibody|Sir2 like 3 antibody|SIR2 like protein 3 antibody|SIR2-like protein 3 antibody|SIR2L3 antibody|SIR3_HUMAN antibody|SIRT 3 antibody|SIRT3 antibody|Sirtuin 3 antibody|Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody|Sirtuin type 3 antibody|Sirtuin3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SIRT3 antibody, ASA-B1695--1.jpg
All lanes: Anti SIRT3 (ASA-B1695) at 0.5ug/ml
WB: Recombinant Human SIRT3 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti SIRT3 (ASA-B1695) at 0.5ug/ml
WB: Recombinant Human SIRT3 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-SIRT3 antibody, ASA-B1695--2.jpg
All lanes: Anti SIRT3 (ASA-B1695) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti SIRT3 (ASA-B1695) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
