More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sex hormone-binding globulin | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SHBG recombinant protein (Position: L30-A210). Human SHBG shares 72% amino acid (aa) sequence identity with both mouse and rat SHBG. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SHBG |
Protein | Sex hormone-binding globulin |
Uniprot ID | P04278 |
Function | Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. |
Tissue Specificity | Isoform 1 and isoform 2 are present in liver and testis. |
Sub-cellular localization | Secreted . Note: In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis. |
Sequence Similarities | Contains 2 laminin G-like domains. |
Aliases | ABP antibody|Androgen binding protein antibody|SBP antibody|Sex hormone-binding globulin antibody|Sex steroid binding protein antibody|Sex steroid-binding protein antibody|SHBG antibody|SHBG_HUMAN antibody|TeBG antibody|Testis specific androgen binding protein antibody|Testis-specific androgen-binding protein antibody|Testosterone binding beta globulin antibody|Testosterone estradiol binding globulin antibody|Testosterone estrogen binding globulin antibody|Testosterone-estradiol-binding globulin antibody|Testosterone-estrogen-binding globulin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SHBG antibody, ASA-B1684, Western blotting
All lanes: Anti SHBG (ASA-B1684) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 70KD
All lanes: Anti SHBG (ASA-B1684) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 70KD
