More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| solute carrier family 5(sodium/glucose cotransporter), member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse SGLT1(620-635aa KMTKEEEEAMKMKMTD), different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SLC5A1 |
Protein | Sodium/glucose cotransporter 1 |
Uniprot ID | P13866 |
Function | Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. |
Tissue Specificity | Expressed mainly in intestine and kidney. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | D22S675 antibody|High affinity sodium glucose cotransporter 1 antibody|High affinity sodium glucose cotransporter antibody|High affinity sodium-glucose cotransporter antibody|Human Na+/glucose cotransporter 1 antibody|Na(+)/glucose cotransporter 1 antibody|Na+/glucose cotransporter 1 antibody|NAGT antibody|SC5A1_HUMAN antibody|SGLT 1 antibody|SGLT1 antibody|SLC5A1 antibody|Sodium glucose cotransporter 1 antibody|Sodium/glucose cotransporter 1 antibody|Solute carrier family 5(sodium/glucose cotransporter) member 1 antibody|Solute carrier family 5 member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SGLT1 antibody, ASA-B1683, Western blotting
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Heart Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: COLO320 Cell Lysate
