More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SGCA recombinant protein (Position: Q24-H387). Human SGCA shares 90.7% amino acid (aa) sequence identity with mouse SGCA. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SGCA |
Protein | Alpha-sarcoglycan |
Uniprot ID | Q16586 |
Function | Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. |
Tissue Specificity | Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain. |
Sub-cellular localization | Cell membrane, sarcolemma ; Single-pass type I membrane protein . Cytoplasm, cytoskeleton . |
Sequence Similarities | Belongs to the sarcoglycan alpha/epsilon family. |
Aliases | 50 DAG antibody|50 kDa dystrophin associated glycoprotein antibody|50 kDa dystrophin-associated glycoprotein antibody|50DAG antibody|50kD DAG antibody| 59kDa antibody|A2 antibody|adhalin antibody|ADL antibody|Alpha SG antibody| Alpha-sarcoglycan antibody|Alpha-SG antibody|Asg antibody|DAG2 antibody| DMDA2 antibody|Dystroglycan 2 antibody|Dystroglycan-2 antibody| LGMD2D antibody|sarcoglycan, alpha (dystrophin-associated glycoprotein) antibody| SCARMD1 antibody|Sgca antibody|SGCA_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SGCA antibody, ASA-B1680, Western blotting
All lanes: Anti SGCA (ASA-B1680) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD
All lanes: Anti SGCA (ASA-B1680) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD
