More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serine/arginine-rich splicing factor 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human SFRS3 (1-29aa MHRDSCPLDCKVYVGNLGNNGNKTELERA), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SRSF3 |
Protein | Serine/arginine-rich splicing factor 3 |
Uniprot ID | P84103 |
Function | May be involved in RNA processing in relation with cellular proliferation and/or maturation. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA- binding activity. RNA-binding is semi-sequence specific. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the splicing factor SR family. |
Aliases | arginine/serine-rich 3 antibody|Pre mRNA splicing factor SRP20 antibody|Pre-mRNA-splicing factor SRP20 antibody|Serine/arginine-rich splicing factor 3 antibody|Splicing factor antibody|Splicing factor arginine/serine rich 20 kD antibody|Splicing factor arginine/ serine rich 3 antibody|SRp20 antibody|SRSF3 antibody|SRSF3_HUMAN antibody|X16 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SFRS3 antibody, ASA-B1677, Western blotting
All lanes: Anti SFRS3 (ASA-B1677) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 19KD
Observed bind size: 19KD
All lanes: Anti SFRS3 (ASA-B1677) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 19KD
Observed bind size: 19KD
