More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| secreted frizzled-related protein 4 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SFRP4 recombinant protein (Position: E140-V346). Human SFRP4 shares 90% and 90.3% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SFRP4 |
Protein | Secreted frizzled-related protein 4 |
Uniprot ID | Q6FHJ7 |
Function | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. |
Tissue Specificity | Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level). |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the secreted frizzled-related protein (sFRP) family. |
Aliases | Frizzled protein antibody|Frizzled protein human endometrium antibody|FRP 4 antibody|FRP4 antibody|FrpHE antibody|human endometrium antibody|Secreted frizzled-related protein 4 antibody|sFRP-4 antibody|Sfrp4 antibody|SFRP4_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SFRP4 antibody, ASA-B1676, Western blotting
All lanes: Anti SFRP4 (ASA-B1676) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti SFRP4 (ASA-B1676) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
