More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| secreted frizzled-related protein 2 | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SFRP2 recombinant protein (Position: L104-C295). Human SFRP2shares99%aminoacid(aa)sequenceidentitywith mouse SFRP2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SFRP2 |
Protein | Secreted frizzled-related protein 2 |
Uniprot ID | Q96HF1 |
Function | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. |
Tissue Specificity | Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. |
Sub-cellular localization | Secreted . |
Sequence Similarities | Belongs to the secreted frizzled-related protein (sFRP) family. |
Aliases | AI851596 antibody|Frizzled related protein 2 antibody|FRP 2 antibody|FRP-2 antibody|FRP2 antibody|MGC128911 antibody|MGC153618 antibody|MGC53344 antibody|SARP 1 antibody|SARP-1 antibody|SARP1 antibody|SDF 5 antibody|SDF5 antibody|Secreted apoptosis related protein 1 antibody|Secreted apoptosis-related protein 1 antibody|Secreted frizzled related protein 2 antibody|Secreted frizzled-related protein 2 antibody|sFRP 2 antibody|sFRP-2 antibody|SFRP2 antibody|Sfrp2 protein antibody|SFRP2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SFRP2 antibody, ASA-B1675, Western blotting
All lanes: Anti SFRP2 (ASA-B1675) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti SFRP2 (ASA-B1675) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
