More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SEMA3A |
Protein | Semaphorin-3A |
Uniprot ID | Q14563 |
Function | Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. |
Tissue Specificity | |
Sub-cellular localization | Secreted . |
Sequence Similarities | Belongs to the semaphorin family. |
Aliases | Coll 1 antibody|Coll1 antibody|HH16 antibody|Hsema I antibody|Hsema III antibody|sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A antibody|Sema III antibody|SEMA1 antibody|SEMA1-PEN antibody|SEMA3A antibody|SEMAD antibody|SEMAIII antibody|SEMAL antibody|Semaphorin 3A precursor antibody|Semaphorin III antibody|Semaphorin like growth cone guidance homolog of chick collapsin antibody|Semaphorin-3A precursor antibody|Semaphorin3A antibody|SemD antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Semaphorin 3A antibody, ASA-B1665, Western blotting
All lanes: Anti Semaphorin 3A (ASA-B1665) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 89KD
Observed bind size: 89KD
All lanes: Anti Semaphorin 3A (ASA-B1665) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 89KD
Observed bind size: 89KD
