SDHA Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
succinate dehydrogenase complex, subunit A, flavoprotein(Fp)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SDHA(113-128aa NMEEDNWRWHFYDTVK), identical to the related rat and mouse sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	SDHA
Protein	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Uniprot ID	P31040
Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Tissue Specificity
Sub-cellular localization	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence Similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
Aliases	CMD1GG antibody|DHSA_HUMAN antibody|Flavoprotein subunit of complex II antibody|Fp antibody|PGL5 antibody|SDH 2 antibody|SDH1 antibody|SDH2 antibody|SDHA antibody|SDHF antibody|Succinate dehydrogenase[ubiquinone] flavoprotein subunit antibody|Succinate dehydrogenase[ubiquinone] flavoprotein subunit mitochondrial antibody|Succinate dehydrogenase[ubiquinone] flavoprotein subunit, mitochondrial antibody|Succinate dehydrogenase complex flavoprotein subunit antibody|Succinate dehydrogenase complex flavoprotein subunit precursor antibody|Succinate dehydrogenase complex subunit A antibody|Succinate Dehydrogenase Complex subunit A Flavoprotein antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information