More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| succinate dehydrogenase complex, subunit A, flavoprotein (Fp) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SDHA recombinant protein (Position: S44-L380). Human SDHA shares 98.2% and 97.6% amino acid (aa) sequence identity with mouse and rat SDHA, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SDHA |
Protein | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial |
Uniprot ID | P31040 |
Function | Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. |
Tissue Specificity | |
Sub-cellular localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. |
Sequence Similarities | Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. |
Aliases | MD1GG antibody|DHSA_HUMAN antibody|Flavoprotein subunit of complex II antibody|FP antibody| PGL5 antibody|SDH 2 antibody|SDH1 antibody|SDH2 antibody|SDHA antibody|SDHF antibody| Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody|Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody|Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody|Succinate dehydrogenase complex flavoprotein subunit antibody|Succinate dehydrogenase complex flavoprotein subunit precursor antibody|Succinate dehydrogenase complex subunit A antibody|Succinate dehydrogenase complex subunit A flavoprotein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- SDHA antibody, ASA-B1658, Western blotting
All lanes: Anti SDHA (ASA-B1658) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: Human Placenta Tissue Lysate at 50ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 73KD
Observed bind size: 73KD
All lanes: Anti SDHA (ASA-B1658) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Mouse Brain Tissue Lysate at 50ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: Human Placenta Tissue Lysate at 50ug
Lane 7: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 73KD
Observed bind size: 73KD
