More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| synaptonemal complex protein 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human SCP3 recombinant protein (Position: E18-E223). Human SCP3 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat SCP3, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SYCP3 |
Protein | Synaptonemal complex protein 3 |
Uniprot ID | Q8IZU3 |
Function | Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity). |
Tissue Specificity | Testis-specific. |
Sub-cellular localization | Nucleus . Chromosome . Note: In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. |
Sequence Similarities | |
Aliases | choline phosphotransferase 1 antibody|chpt1 antibody|COR 1 antibody|COR1 antibody|MGC71888 antibody|RNASCP3 antibody|SCP 3 antibody|SCP-3 antibody|SCP3 antibody|SPGF4 antibody|Sycp 3 antibody|Sycp3 antibody|SYCP3_HUMAN antibody|Synaptonemal complex protein 3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-SCP3 antibody, ASA-B1654--1.jpg
All lanes: Anti SCP3 (ASA-B1654) at 0.5ug/ml
WB: Recombinant Human SCP3 Protein 0.5ng
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti SCP3 (ASA-B1654) at 0.5ug/ml
WB: Recombinant Human SCP3 Protein 0.5ng
Predicted bind size: 41KD
Observed bind size: 41KD
Anti-SCP3 antibody, ASA-B1654--2.jpg
All lanes: Anti SCP3 (ASA-B1654) at 0.5ug/ml
WB: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
All lanes: Anti SCP3 (ASA-B1654) at 0.5ug/ml
WB: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
