More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| runt-related transcription factor 3 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse RUNX3(186-205aa QKIEDQTKAFPDRFGDLRMR), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RUNX3 |
Protein | Runt-related transcription factor 3 |
Uniprot ID | Q64131 |
Function | Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Cytoplasm . Note: The tyrosine phosphorylated form localizes to the cytoplasm. |
Sequence Similarities | |
Aliases | Acute myeloid leukemia 2 protein antibody|Acute myeloid leukemia gene 2 antibody|AML 2 antibody|AML2 antibody|CBF alpha 3 antibody|CBF-alpha-3 antibody|CBFA 3 antibody|CBFA3 antibody|Core binding factor alpha 3 subunit antibody|core binding factor antibody|Core binding factor runt domain alpha subunit 3 antibody|Core binding factor subunit alpha 3 antibody|core-binding factor antibody|Core-binding factor subunit alpha-3 antibody|FLJ34510 antibody|MGC16070 antibody|Oncogene AML 2 antibody|Oncogene AML-2 antibody|PEA2 alpha C antibody|PEA2-alpha C antibody|PEBP2 alpha C antibody|PEBP2-alpha C antibody|Pebp2a3 antibody|PEBP2aC antibody|Polyomavirus enhancer binding protein 2 alpha C subunit antibody|Polyomavirus enhancer-binding protein 2 alpha C subunit antibody|runt domain alpha subunit 3 antibody|runt related transcription factor 3 antibody|Runt-related transcription factor 3 antibody|RUNX 3 antibody|Runx3 antibody|RUNX3_HUMAN antibody|SL3 3 enhancer factor 1 alpha C subunit antibody|SL3-3 enhancer factor 1 alpha C subunit antibody|SL3/AKV core binding factor alpha C subunit antibody|SL3/AKV core-binding factor alpha C subunit antibody|Transcription factor AML2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- RUNX3 antibody, ASA-B1641, Western blotting
All lanes: Anti RUNX3 (ASA-B1641) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 44KD
Observed bind size: 44KD
All lanes: Anti RUNX3 (ASA-B1641) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 44KD
Observed bind size: 44KD
