More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| receptor-interacting serine-threonine kinase 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human RIP3(60-76aa EVKAMASLDNEFVLRLE). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RIPK3 |
Protein | Receptor-interacting serine/threonine-protein kinase 3 |
Uniprot ID | Q9Y572 |
Function | Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production. |
Tissue Specificity | Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. |
Sub-cellular localization | Cytoplasm, cytosol. |
Sequence Similarities | Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. |
Aliases | Receptor interacting protein 3 antibody|Receptor interacting serine threonine kinase 3 antibody|Receptor interacting serine/threonine protein kinase 3 antibody|Receptor-interacting protein 3 antibody|Receptor-interacting serine/threonine-protein kinase 3 antibody|RIP 3 antibody|RIP like protein kinase 3 antibody|RIP-3 antibody|RIP-like protein kinase 3 antibody|RIPK 3 antibody|RIPK3 antibody|RIPK3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-RIP3 antibody, ASA-B1632, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
Lane 1: PANC Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: SKOV-3 Cell Lysate
Lane 4: M231 Cell Lysate
