Rb2 p130 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
retinoblastoma-like 2(p130)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rb2 p130(1120-1139aa ENHSALLRRLQDVANDRGSH), identical to the related rat sequence and different from the related mouse sequence by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	RBL2
Protein	Retinoblastoma-like protein 2
Uniprot ID	Q08999
Function	Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Tissue Specificity
Sub-cellular localization	Nucleus.
Sequence Similarities	Belongs to the retinoblastoma protein (RB) family.
Aliases	130 kDa retinoblastoma associated protein antibody|FLJ26459 antibody|p130 antibody|PRB 2 antibody|PRB2 antibody|Rb 2 antibody|Rb2 antibody|RBL 2 antibody|RBL2 antibody|RBR 2 antibody|RBR2 antibody|Retinoblastoma like 2 antibody|Retinoblastoma like protein 2 antibody|Retinoblastoma related gene antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information