More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| retinoblastoma-like 2(p130) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Rb2 p130(1120-1139aa ENHSALLRRLQDVANDRGSH), identical to the related rat sequence and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RBL2 |
Protein | Retinoblastoma-like protein 2 |
Uniprot ID | Q08999 |
Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the retinoblastoma protein (RB) family. |
Aliases | 130 kDa retinoblastoma associated protein antibody|FLJ26459 antibody|p130 antibody|PRB 2 antibody|PRB2 antibody|Rb 2 antibody|Rb2 antibody|RBL 2 antibody|RBL2 antibody|RBR 2 antibody|RBR2 antibody|Retinoblastoma like 2 antibody|Retinoblastoma like protein 2 antibody|Retinoblastoma related gene antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Rb2 p130 antibody, ASA-B1615, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: NIH313 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: NIH313 Cell Lysate
