More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine (C-C motif) ligand 5 | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human RANTES recombinant protein (Position: Y26-S91). Human RANTES shares 83.3% amino acid (aa) sequence identity with both mouse and rat RANTES. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CCL5 |
Protein | C-C motif chemokine 5 |
Uniprot ID | P13501 |
Function | Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1- infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1- 68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:16791620, PubMed:1380064, PubMed:8525373, PubMed:9516414, PubMed:15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485). |
Tissue Specificity | T-cell and macrophage specific. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine beta (chemokine CC) family. |
Aliases | Beta chemokine RANTES precursor antibody|CCL 5 antibody|CCL5 antibody|CCL5_HUMAN antibody|Chemokine (C C motif) ligand 5 antibody| Chemokine CC Motif Ligand 5 antibody|D17S136E antibody|EoCP antibody|Eosinophil chemotactic cytokine antibody|MGC17164 antibody| RANTES(4-68) antibody|Regulated upon activation normally T expressed and presumably secreted antibody|SCYA 5 antibody|SCYA5 antibody| SIS delta antibody|SIS-delta antibody|SISd antibody|Small inducible cytokine A5 (RANTES) antibody|Small inducible cytokine A5 antibody|Small inducible cytokine subfamily A (Cys Cys) member 5 antibody|Small-inducible cytokine A5 antibody|T cell specific protein p288 antibody|T cell specific RANTES protein antibody|T cell-specific protein P228 antibody|T-cell-specific protein RANTES antibody|TCP 228 antibody|TCP228 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-RANTES antibody, ASA-B1611, Western blotting
All lanes: Anti RANTES (ASA-B1611) at 0.5ug/ml
WB: HUT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti RANTES (ASA-B1611) at 0.5ug/ml
WB: HUT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
