More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| RAB11A, member RAS oncogene family | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Rab11A(194-211aa NVVPIHVPPTTENKPKVQ), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | RAB11A |
Protein | Ras-related protein Rab-11A |
Uniprot ID | P62491 |
Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Cleavage furrow. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane ; Lipid-anchor ; Cytoplasmic side . Note: Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Recruited to phagosomes containing S.aureus or M.tuberculosis. |
Sequence Similarities | |
Aliases | MGC1490 antibody|Rab 11 antibody|Rab 11A antibody|RAB 11A member oncogene family antibody|RAB 11A, member oncogene family antibody|Rab-11 antibody|RAB11 A antibody|RAB11 antibody|RAB11A antibody|RAB11A member RAS oncogene family antibody|Ras related protein Rab 11A antibody|Ras related protein Rab11A antibody|Ras-related protein Rab-11A antibody|YL 8 antibody|YL8 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Rab11A antibody, ASA-B1597, All Western blotting
All lanes: Anti-RAB11A(ASA-B1597) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Lane 3: A431 Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
All lanes: Anti-RAB11A(ASA-B1597) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: NIH Whole Cell Lysate at 40ug
Lane 3: A431 Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD
